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3 R's - History
The so-called "three R's" of animal research - replacement, reduction and refinement - were first proposed by William Russell, a zoologist, and microbiologist Rex Burch. In the mid-1950s, they were hired by the Universities Federation for Animal Welfare, a scientific animal welfare organization based in the United Kingdom, to conduct a study of humane techniques for laboratory animal experiments.   After five years of research, Russell and Burch grouped their findings under the headings of replacement, reduction and refinement. They maintained that those involved with laboratory animal use have a moral duty to seek replacements to animal experiments whenever possible, keep the numbers of animals used at a minimum and refine procedures to lessen discomfort and stress.

They also maintained that good science and animal welfare were inextricably linked. Chronic stress in research animals could result in inaccurate and misleading experimental data, further supporting their call for humane treatment. They wrote:

If we are to use a criterion for choosing experiments to perform, the criterion of humanity is the best we could possibly invent. The greatest scientific experiments have always been the most humane and the most aesthetically attractive, conveying that sense of beauty and elegance which is the essence of science at its most successful.

They published their findings in 1959 in The Principles of Humane Experimental Technique. These tenets underpin most animal research policy and practice today. They have been incorporated into various U.S. and international laws and embraced by a variety of organizations around the world such as the Center for Alternatives to Animal Testing at Johns Hopkins University and the European Centre for the Validation of Alternative Methods, based in Ispra, Italy.

The full text of The Principles of Humane Experimental Technique can be viewed on the Altweb site maintained by the Center for Alternatives to Animal Testing, Johns Hopkins University.